Use of control sera for monitoring temperature changes during pH and pCO2 measurements.

hydrochloride (10 mg three times a day for as long as 20 days) did not affect values for 17-hydroxycorticosteroids. In vitro data do show that there is a small increase in the apparent value for urinary 17-hydroxycorticosteroids when chlordiazepoxide or its lactam or N-despiethyl metabolite is added to urine. However, this increase does not appear to be large enough to be of significance. The fact that this small increase is not corroborated by the in vivo data may be explained by intrapatient variation from day to day. There was no decrease in the values for 17-ketosteroids either in vivo or in vitro, in contrast to previously reported results. In serum analyses-including aspartate aminotransfer-ase, alkaline phosphatase, bilirubin, albumin, total protein, and protein electrophoresis-with use of as much as 10-fold the therapeutic blood concentrations of chlordiazepox-ide and 200-fold the blood concentrations of the major me-tabolites, we saw no in vitro effect of the addition of chlor-diazepoxide or either of its metabolites. No in vivo data for serum are presented here. These data will be included in a future study on drug interference. Jaundice after therapy with chlordiazepoxide hydrochloride. necrosis after chlordiazepoxide therapy. interfere with routine endocrine laboratory procedures. Clin. of a dye-binding method for determination of serum albumin. A continuous spectro-photometric method for measuring the activity of serum alkaline phosphatase. spectrophotometric methods for the determination of glutamic-oxalacetic glutamic-pyruvic transaminase, and lactic acid dehydro-genase. Amer.droxy-20-ketosteroids in urine and plasma. An improved method for assaying the steroidogenic potency of ACTH. Zimmerman reagent for the determination of 17-ketosteroid reaction. of a 17-ketosteroid reaction. The determination of urinary steroids. (1) The preparation of pigment free extracts and a simplified procedure for estimation of total 17-ketosteroids. of chlordiazepoxide.tion of creatinine by means of an automatic chemical analyzer. We evaluated the temperature coefficients from 25 to 38 #{176}C for aqueouscalibrationmaterials,serum(protein-based) control material, and patients' samples in blood pH measurements and gas analysis. Whereas the aque-ous buffers and calibration gases (used as unknowns) were not affected by changing temperature, the temperature coefficients of patients' samples and protein-based control materials varied similarly to those reported in the literature: 0.011 and 0.014 vs. 0.015 pH/ #{176}C, and 1.76 and 1.57 vs. 1.80 mm Hg/#{176}C. We conclude that the periodic use of suitable control materials can assist in the detection of temperature abnormalities. Temperature is a critical parameter in blood pH measurement and gas analysis. Reported temperature coefficients of patients' blood samples …